ABSTRACT
Objective To understand the situation of seroepidemiology of Toxoplasma gondii infection among employees in a pork food processing enterprise in Dongtai City. Methods Totally 200 employees from a pork food processing enterprise in Dongtai City were selected as the research objects. The venous blood of the selected population was extracted and the IgG antibody against T. gondii in serum was detected by ELISA, and the results were analyzed. Results Among the 200 employees, 36 cases were positive in IgG antibody detection, the positive rate was 18.0%. The positive rate of IgG antibody among workers with different length of service was significantly different (χ2 = 9.813, P < 0.05). With the increase of working age, the positive rate of IgG antibody gradually increased. The positive rate of IgG antibody to T. gondii was significantly higher in the population who had frequent contact with animals. Conclusions The positive rate of IgG antibody against T. gondii in employees in pork food processing enterprises is high. Therefore, the detection of antibodies against T. gondii and health education should be strengthened in this population.
ABSTRACT
Objective To understand the situation of seroepidemiology of Toxoplasma gondii infection among employees in a pork food processing enterprise in Dongtai City. Methods Totally 200 employees from a pork food processing enterprise in Dongtai City were selected as the research objects. The venous blood of the selected population was extracted and the IgG antibody against T. gondii in serum was detected by ELISA, and the results were analyzed. Results Among the 200 employees, 36 cases were positive in IgG antibody detection, the positive rate was 18.0%. The positive rate of IgG antibody among workers with different length of service was significantly different (χ2 = 9.813, P < 0.05). With the increase of working age, the positive rate of IgG antibody gradually increased. The positive rate of IgG antibody to T. gondii was significantly higher in the population who had frequent contact with animals. Conclusions The positive rate of IgG antibody against T. gondii in employees in pork food processing enterprises is high. Therefore, the detection of antibodies against T. gondii and health education should be strengthened in this population.
ABSTRACT
<p><b>AIM</b>To investigate the level of immune response and the immune mechanism of the single-dose hepatitis B surface antigen (HBsAg)-poly (d, l)-lactide-co-glicolide acid (PLGA) microspheres in BALB/c mice.</p><p><b>METHODS</b>Three kind of HBsAg-PLGA microspheres, HBsAg-PLGA50/50-COOH microspheres, HBsAg-PLGA75/25 microspheres and HBsAg-PLGA50/50 microspheres, were prepared by double emulsion microencapsulation technique used three kinds of PLGA with different L/G ratio. The single-dose of HBsAg-PLGA microspheres was subcutaneously injected into BALB/c mice at the dose of 7.5 microg HBsAg per mouse. The conventional aluminum-adjuvant vaccine was subcutaneously injected at 0, 1 and 2 month as positive control. In certain time interval, the induced immune level of total antibody was detected by enzyme linked immunosorbent assay (ELISA). For subclass of IgG antibody and cytokines studies, the dose of HBsAg was 2.5 microg per mouse.</p><p><b>RESULTS</b>The HBsAg-PLGA microspheres could successfully induce a humoral immune response in BALB/c mice. Compared with the conventional aluminum-adjuvant vaccine, the antibody response of the HBsAg-PLGA50/50-COOH microspheres was significantly lower than the group received three injections of aluminum-adjuvant vaccine (P < 0.01) except for a higher priming response during the early 6 weeks. The results were ascribed to the relatively rapid degradation charactics of PLGA50/50-COOH polymer. The immune response for the HBsAg-PLGA50/50 microspheres and HBsAg-PLGA75/25 microspheres were comparable to the group administered with aluminum-adjuvant vaccine (P > 0.05) which was due to the sustained degradation of PLGA50/50 and PLGA75/25 polymer.</p><p><b>CONCLUSION</b>The HBsAg-PLGA microsphere is a promising candidate for the controlled delivery of a vaccine which does not require multiple injections.</p>
Subject(s)
Animals , Female , Mice , Rats , Delayed-Action Preparations , Dose-Response Relationship, Immunologic , Drug Carriers , Hepatitis B Antibodies , Blood , Hepatitis B Surface Antigens , Chemistry , Allergy and Immunology , Hepatitis B Vaccines , Allergy and Immunology , Immunization , Immunoglobulin G , Blood , Interferon-gamma , Metabolism , Interleukin-2 , Metabolism , Interleukin-5 , Metabolism , Lactic Acid , Mice, Inbred BALB C , Microspheres , Polyglycolic Acid , Polymers , Random Allocation , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>This clinical study was to improve the surgical treatment to craniomaxillofacial tissue defects.</p><p><b>METHODS</b>Since 1997, eight cases with severe craniomaxillofacial defects were treated using free latissimus dorsi myocutaneous flaps. In the operation, nerve anastomosis was performed. Of the 8 cases, 7 were treated in one stage, 1 was treated in 3 steps. The craniomaxillofacial defects ranged from 10 cm x 8 cm to 30 cm x 12 cm. The flaps was 12 cm x 10 cm to 32 cm x 16 cm in size.</p><p><b>RESULTS</b>Postoperative follow-up for 6 months to 4 years demonstrated satisfactory results in all the cases. There was neither necrosis nor ulcer after the operation. The sensation recovery of the flap was also satisfactory.</p><p><b>CONCLUSION</b>Free transfer of the latissimus dorsi myocutaneous flap is an ideal treatment to severe craniomaxillofacial defects as it possesses the advantages of reliable blood supply, ability against infections, large size, concealed donor site, and functional restoration of sensation and movement.</p>